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. 2004 Sep 1;18(17):2147–2160. doi: 10.1101/gad.302904

Figure 3.

Figure 3.

The relocation of TIF1β from eu- to heterochromatin is not required for PrE differentiation. (A) Wild-type (WT), TIF1β+/-, and TIF1βHP1box/- F9 cells were grown on glass coverslips, treated with 1 μM tRA for 4 d, fixed, and hybridized with an anti-Troma-1 mAb as indicated. Nuclei were visualized by Hoechst staining. Projection of three confocal sections through nondifferentiated and differentiated wild-type, TIF1β+/-, and TIF1βHP1box/- F9 cells is shown. Bar, 50 μm. (B) TIF1βHP1box does not relocate from eu- to heterochromatin during PrE differentiation. TIF1β+/- and TIF1βHP1box/- were grown on glass coverslips for 4 d in the presence or absence of 1 μM tRA, fixed, and hybridized with an anti-Troma-1 mAb and the anti-TIF1β pAb PF64. The DNA content was visualized by Hoechst staining. Single confocal sections through differentiated (panels g–l) and nondifferentiated cells (panels a–f) are shown. Bars, 5 μm.