Fig 6. Yield of singlet oxygen and hyperthermia effect of HIPPNPs under NIR laser irradiation.
Upon exposed to 808-nm CW laser with intensity of 6 W/cm2, both production of singlet oxygen (A) and variation of temperature (B) generated from the HIPPNPs with 0, 1.25, 2.5, 5, 10, and 25 μM ICG equivalent and freely dissolved ICG with concentrations of 2.5 and 25μM were measured every 60 (for yield of singlet oxygen) or 30 (for temperature change) sec for 5 min. The quantity of singlet oxygen was represented as the intensity of SOSG-induced fluorescence which was measured by spectrofluorometry with 488 and 525 nm of excitation and emission wavelength, respectively. The temperature at each time point was measured by using a digital thermometer. Values are mean ± SD (n = 3) in both examinations.