Figure 1.

Schematic representation of the imprinted mouse distal 12 domain (human 14q32). The position of several imprinted genes is indicated by squares (Gtl2 and protein-coding genes), vertical bars (snoRNA genes), or triangles (miRNA genes). Maternally expressed and paternally expressed genes are filled in pink and blue, respectively. Biallelically expressed genes and genes with undetermined imprinted status are colored in black and grey, respectively. Open triangles represent in silico-predicted miRNA gene that we failed to experimentally detect (i.e., pre-mir-L, pre-mir-H, pre-mir-G, pre-mir-I, pre-mir-Q). Two recently described miRNA genes—mir-342 and mir-345 (Kim et al. 2004)—map within an intron of Ev1 and between Yy1 and Wars genes, respectively. Imprinting status of mir-342 remains unsolved as we failed to detect it in embryos. An intergenic germ-line derived differentially methylated region (IG-DMR) located between Dlk1 and Gtl2 genes is represented by circles (filled indicates hypermethylated; open, hypomethylated). Mat indicates maternal chromosome; Pat, paternal chromosome. The figure is not drawn to scale.