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. 2004 Sep;14(9):1749–1755. doi: 10.1101/gr.2833204

Figure 2.

Figure 2

Results of single base extension reaction using synthetic oligos with or without PPi and with or without pyrophosphatase. (A) Results of primer extension reaction in the absence of PPi. No obvious misincorporation was observed up to 70 thermal cycles. (B) Results of primer extension reaction in the presence of PPi. The misincorporation of R110-G for homozygotes was evident after only 10 cycles, causing the merging of the CC cluster and the GC cluster. (C) Results of the primer extension reaction after incubation with pyrophosphatase to remove the added PPi. No evidence of misincorporation up to 70 cycles.