Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Dec 7;11:6353–6364. doi: 10.2147/IJN.S92608

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2016 Kasper et al. This work is published and licensed by Dove Medical Press Limited

The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

PMC Copyright notice

Immunofluorescence and TEM of the Caco/2/ISO-HAS-1 coculture.

Notes: ([A], a1 and a2) Immunofluorescence staining of tight junctional proteins (ZO-1, red signal [A], a1) and adherens junctional proteins (beta-catenin, green signal [A], a2) of Caco-2 after 21 days in coculture with ISO-HAS-1 on the opposite side of the filter membrane. ZO-1 and beta-catenin appeared well developed. TEM images corroborate these observations, desmosomal ([C] c1, arrows) as well as tight junctional ([C] c2, arrows) complexes appear well defined. (B) CD31 staining of the ISO-HAS-1 cells underneath the Caco-2 layer. A well-developed endothelial monolayer could be detected. Nuclei (blue signal, Hoechst 33342) scale bar for fluorescence images (A), a1 and a2: 5 μm; (B) 15 μm. (C) TEM cross-section through a transwell filter membrane with Caco-2 on the top side and ISO-HAS-1 on the bottom side (magnifications C: 2,000×; c1, c2: 20,000×).

Abbreviations: TEM, transmission electron microscopy; ZO-1, zona occludens-1.