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. 2016 Dec 7;11:6353–6364. doi: 10.2147/IJN.S92608

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© 2016 Kasper et al. This work is published and licensed by Dove Medical Press Limited

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TER of Caco-2 in monoculture (dashed line) and coculture with ISO-HAS-1 (continuous line) after 21 days in culture.

Notes: (A) The Caco-2 monoculture developed a significant higher TER compared to the Caco-2 in coculture, but it is still high enough and sufficient to influence external impact on barrier integrity. (B) The transport-assay (P_app) using NaFITC revealed comparable results in the Caco-2 monoculture as well as coculture with ISO-HAS-1. Data are shown as mean ± SD from at least three separate experiments. Testing for significant differences between means was carried out using one-way ANOVA and Dunnett’s Multiple Comparison test at a probability of error of 0.1% (***).

Abbreviations: ANOVA, analysis of variance; LPS, lipopolysaccharide; SD, standard deviation; TER, transepithelial electrical resistance; uc, untreated control.