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. Author manuscript; available in PMC: 2016 Dec 12.
Published in final edited form as: Biochim Biophys Acta. 2010 Jan 14;1799(5-6):469–479. doi: 10.1016/j.bbagrm.2010.01.005

Fig. 6.

Fig. 6

RNAi-mediated knockdown of ERα in T-47D cells. (A) T-47D cells were transfected with either siRNA targeting luciferase (siLuc) or two different siRNAs targeting ERα (siERα-11 or siERα-14) for 48 h. RNA was isolated and CYP2B6 and ERα expression levels were analyzed by qPCR as described in Materials and methods. CYP2B6 mRNA levels were normalized to non-transfected T-47D cells (control), which was set to 1. Statistical significance (p<0.05 Student’s t-test) compared to non-targeting pool is indicated by an asterisk. Results shown represent the means±S.E.M. for three independent experiments. (B) T-47D cells were transfected with either non-targeting pool reagent or siRNA against ERα for 48 h. ChIP assays were performed and recruitment levels are presented as a percentage of a 5% total chromatin input. Statistical significance (p<0.05 Student’s t-test) compared to non-targeting pool is indicated by an asterisk. Results shown are the means± S.E.M. for three independent experiments.