Skip to main content
. 2004 Sep;15(9):4011–4022. doi: 10.1091/mbc.E03-12-0876

Figure 3.

Figure 3.

Fab fragments derived from syntaxin 5 or GS15 antibodies inhibited the EE/RE-TGN transport. Fab fragments were prepared from syntaxin 5 or GS15 antibodies. Transport was carried out in the presence of buffer (controls), Fab fragments, antigens, or heat-inactivated Fab fragments. Where both Fab fragments and antigens were present, Fab fragments were incubated with antigens on ice for 1 h before being added to the transport reactions. The extent of transport relative to each control (defined as 100%) was calculated from two to three separate experiments. (A) Transport in the presence of the Fab fragments and/or antigens of syntaxin 5 antibodies. (B) Transport in the presence of the Fab fragments and/or antigens of GS15 antibodies. Error bars represented standard deviations. The respective concentrations (micrograms per milliliter) of the added reagents were shown in brackets. H, heat inactivated.