Figure 7.

Syntaxin 16-mediated (syntaxin 5-independent) and syntaxin 5-mediated (syntaxin 16-independent) routes have similar time courses of transport. Transport reactions with either 67 μg/ml syntaxin 5 or 20 μg/ml syntaxin 16 antibodies were allowed to proceed for 10, 15, 20, 30, 40, 50, 60, and 70 min at 37°C and stopped on ice. Controls (cont.) were transport reactions that proceeded for 70 min in the absence of any antibody. Transport relative to control (set as 100%) at each time point was calculated. (A) Representative phosphorimaging data. (B) Graph plotted from duplicates. Curves fitting each set of data are presented.