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Western blot detection of stably expressed proteins. The α1 and β1 subunits of NO-sensitive GC and human PDE5 were detected in HEK 293 cells after stable transfection of the respective cDNAs. Untransfected control cells (HEK) are shown in the first lane; lanes 2-6 contain the cell lines used in this study. The β1 subunit of HEK-GClow cells is below the detection limit but becomes visible upon longer exposure.
