Figure 2.
NO-induced cGMP accumulation in HEK cells stably expressing NO-sensitive GC. (A) cGMP response in untransfected control cells (HEK) and in cells with high (HEK-GC) and low (HEK-GClow) expression level of NO-sensitive GC after stimulation with GSNO (100 μM) in the absence or presence of YC-1 (100 μM). (B) Relative GTP content of HEK-GC cells 5 min after the incubation with or without GSNO (left). GTP was quantified by determination of area under the curve after chromatographic separation of nucleotides using a Mono-Q column. Right, original trace data of a representative elution profile.