Figure 2.

Characterization of CD147 glycosylation. (A) HT1080 cells were treated with 0, 5, or 10 μg/ml tunicamycin for 12 h. CD147 immunoprecipitates were then blotted for CD147 (top) or associated caveolin-1 (middle). Caveolin-1 in total cell lysates was also blotted (bottom). (B) CD147 was immunoprecipitated from HT1080 cells (with mAb 8G6), and samples were then treated with 1, 5, or 10 μl of endo H (1000 U/μl), in 1.0 ml of buffer, at 37°C for 30 min before SDS-PAGE. CD147 was then detected by blotting with polyclonal antibody B10. IP, immunoprecipitated; IB, immunoblotted.