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Caveolin-1 effects on CD147 biosynthesis. In control HEK293 cells or cells stably expressing caveolin-1, endogenous CD147 was labeled with [35S]methionine/cysteine for 1 h, immunoprecipitated, and detected as in Figure 3B. After the 1-h pulse, the 35S-label was chased for the additional times indicated, in medium containing 5.0% dialyzed fetal calf serum and 25× excess unlabeled l-methionine.
