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Glo3-R59K coimmunoprecipitates with the coatomer complex. Protein extracts from glo3Δ mutant cells expressing Glo3-R59K were incubated with anti-Sec21 antibody, followed by incubation with protein A-agarose beads. Beads were pelleted by centrifugation, washed, and resuspended in 1× Laemmli buffer. Samples were run on a 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane. The blot was decorated with Glo3 antibodies.
