Table 1.
Media composition
| Media composition | Batch Medium | Chemostat medium |
|---|---|---|
| Phosphate | 13.3 mg/l KH2PO4 (98 uM) | 13.3 mg/l KH2PO4 (98 uM) |
| 0.1 g/l Calcium chloride | 0.1 g/l Calcium chloride | |
| 0.1 g/l Sodium chloride | 0.1 g/l Sodium chloride | |
| 0.5 g/l Magnesium sulfate | 0.5 g/l Magnesium sulfate | |
| 0.1 g/l Potassium chloride | 0.1 g/l Potassium chloride | |
| 5 g/l Ammonium sulfate | 5 g/l Ammonium sulfate | |
| 10 g/l Glucose | 10 g/l Glucose | |
| 1 ml/l 1000× Vitamins | 1 ml/l 1000× Vitamins | |
| 1 ml/l 1000× Metals | 1 ml/l 1000× Metals | |
| Sulfate | 3 mg/l Ammonium sulfate (22.7 uM) | 3 mg/l Ammonium sulfate (22.7 uM) |
| 0.1 g/l Calcium chloride | 0.1 g/l Calcium chloride | |
| 0.1 g/l Sodium chloride | 0.1 g/l Sodium chloride | |
| 0.412 g/l Magnesium chloride | 0.412 g/l Magnesium chloride | |
| 1 g/l KH2PO4 | 1 g/l KH2PO4 | |
| 4.05 g/l Ammonium chloride | 4.05 g/l Ammonium chloride | |
| 10 g/l Glucose | 10 g/l Glucose | |
| 1 mL/l 1000× Vitamins | 1 mL/l 1000× Vitamins | |
| 1 mL/l 1000× Metals | 1 mL/l 1000× Metals | |
| Leucine | 40 mg/l Leucine (305 uM) | 15 mg/l Leucine (114 uM) |
| 0.1 g/l Calcium chloride | 0.1 g/l Calcium chloride | |
| 0.1 g/l Sodium chloride | 0.1 g/l Sodium chloride | |
| 0.5 g/l Magnesium sulfate | 0.5 g/l Magnesium sulfate | |
| 1 g/l KH2PO4 | 1 g/l KH2PO4 | |
| 5 g/l Ammonium sulfate | 5 g/l Ammonium sulfate | |
| 10 g/l Glucose | 10 g/l Glucose | |
| 1 mL/l 1000× Vitamins | 1 mL/l 1000× Vitamins | |
| 1 mL/l 1000× Metals | 1 mL/l 1000× Metals | |
| Uracil | 6 mg/l Uracil (53.5 uM) | 5 mg/l Uracil (44.6 uM) |
| 0.1 g/l Calcium chloride | 0.1 g/l Calcium chloride | |
| 0.1 g/l Sodium chloride | 0.1 g/l Sodium chloride | |
| 0.5 g/l Magnesium sulfate | 0.5 g/l Magnesium sulfate | |
| 1 g/l KH2PO4 | 1 g/l KH2PO4 | |
| 5 g/l Ammonium sulfate | 5 g/l Ammonium sulfate | |
| 10 g/l Glucose | 10 g/l Glucose | |
| 1 mL/l 1000× Vitamins | 1 mL/l 1000× Vitamins | |
| 1 mL/l 1000× Metals | 1 mL/l 1000× Metals | |
| Stock solutions | ||
| Trace metals | 500 mg Boric acid | |
| Stock solution, 1000× | 40 mg Copper sulfate. 5H2O | |
| 100 mg Potassium iodide | ||
| 200 mg Ferric chloride. 6H2O | ||
| 400 mg Maganese sulfate. H2O | ||
| 200 mg Sodium molybdate. 2H2O | ||
| 1 l Deionized water, q.s. | ||
| Vitamins | 1 mg Biotin | |
| Stock solution, 1000× | 200 mg Calcium pantothenate | |
| 1 mg Folic acid | ||
| 1000 mg Inositol | ||
| 200 mg Niacin (nicotinic acid) | ||
| 100 mg P-aminobenzoic acid | ||
| 200 mg Pyridoxine HCl | ||
| 100 mg Riboflavin | ||
| 200 mg Thiamine HCl | ||
| 500 mL Water |