Fig. 3.

The effect of the timing of caffeic acid treatment in combination with cisplatin in A2780 cells. (a) Co-administration of cisplatin and 50 µM of caffeic acid or caffeic acid and trigonelline for 24 h. (b) 6 h pre treatment of caffeic acid or caffeic acid and trigonelline followed by washout and cisplatin treatment for additional 24 h. Cisplatin treatment (black); combination of cisplatin and caffeic acid (white); combination of cisplatin, caffeic acid and trigonelline (grey). Results are presented as the means of cytotoxicity±SD. * indicates signnificance (p<0.05) comparing to treatment of cisplatin control of the same concentration or as indicated in the figure.