Figure 5.

Affinity of rhesus immunoglobulin G (IgG) for low affinity human FcγR. (A,B) Equilibrium binding affinity (K_D in micromolar) of recombinant monoclonal rhesus IgG subclasses [Macaca mulatta (MM) IgG1–4] as well as a commercially supplied sample of purified polyclonal rhesus macaque plasma IgG (MM pIgG) to the low affinity human FcγR. Bars denote the mean of two to six replicates, in two separate experiments (upper and lower panels) using different preparations of FcγR. (B) Equilibrium binding affinity (K_D in micromolar) of 10 randomly selected purified rhesus macaque plasma IgG samples. Bars and whiskers denote the median and interquartile range.