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. 2016 Dec 6;111(11):2440–2449. doi: 10.1016/j.bpj.2016.10.016

Figure 3.

Figure 3

αS concentration dependent changes in DNBD-PC in POPC: POPG (50:50) SLBs. Incubation of increasing concentrations of monomeric αS, or increased P/L ratios consistently resulted in a drop in the DNBD-PC after protein addition for all αS variants except Δ71-82-αS (red circles).The decrease in the DNBD-PC is more pronounced for the 1-108-αS (green downward triangles) and 1-60-αS (magenta upward triangles) as compared with WT-αS (black squares) for all P/L ratios. The error bars indicate standard deviations obtained from five individual FRAP measurements. All experiments were carried out in 50 mM HEPES buffer, 0.1 mM EDTA (pH 7.4) at room temperature. To see this figure in color, go online.