Figure 4.

Structural dynamics in the proposed copper entry site. (A) The dynamic side-chain reorientation between ion entry site Met148 and the internal ion acceptor Cys382 visualized by two superimposed structures that represent extreme configurations with respect to the Cys382-Met148 distance (minimal (4.3 Å) and maximal (15 Å)). (B) The simulated variations in center-of-mass of residues Met148 and Cys382 in the three Cu⁺-free simulations. (C) Relative in vitro (first columns) and in vivo (second columns) activities of LpCopA entry/transition site mutants as compared to wild-type (WT) protein and the inactive Asp426Asn mutant. To see this figure in color, go online.