Figure 6.

(A) In identifying a fibril spectral component in the temperature-dependent fibrillogenesis of Telo-EPC, analysis indicated that three components were sufficient to recover experimental spectra at all temperatures (χ²/N = 2.0 for N = 3). (B) In addition to constrained helix and coil components, a fibril spectral component was identified with a negative band at 210 nm for this experiment. (C) The fraction of signal of the three species as a function of temperature reveals two transitions—from helix to fibril, and from fibril to coil—as previously identified in the Telo-EPC temperature melt data (Fig. 1A).