Table 2.
Mean distances of Tpr domains from the NE midplane and the central symmetry axis of the NPC in FA/GA-fixed specimen
| Distance NE midplaneb (x-axis) | Distance NE midplane [normalized]c | Distance NPC-axis (y-axis)d | Distance NPC-axis [normalized]e | |||||
|---|---|---|---|---|---|---|---|---|
| Antibody | Sample sizea | (Mean) | (Mean) | (SE) | Sample sized | (Mean) | (Mean) | (SE) |
| Tpr (636-655)f | 101/93 | -33.0* | -35.4* | 2.3 | 62 | 23.0 | 35.0 | 1.2 |
| Tpr (914-932) | 92/90 | -58.7 | -62.4 | 2.6 | 65 | 16.1 | 32.6 | 0.8 |
| Tpr (2063-2084) | 179/165 | -70.5 | -73.9 | 1.8 | 65 | 13.7 | 30.8 | 1.0 |
| Tpr (2338-2363) | 121/117 | -73.2 | -76.1 | 2.1 | 63 | 12.6 | 27.8 | 1.0 |
Cells were fixed in 2% formaldehyde plus 0.1% glutaraldehyde.
Data collection was as described for Table 1. Boundaries between which gold grains located in proximity to perpendicularly sectioned NPCs were measured had initially been set at a distance of 100 nm from the NE midplane on its cytoplasmic side and 200 nm on its nuclear side (sample size values on the left). For further calculations of mean distances only gold grains located up to 150 nm on the nuclear side of the NE were considered (sample size values on the right).
The negative algebraic sign of an NE distance value (given in nanometer) indicates nuclear localization.
Normalization of values for NE width variations as described in Figure 3A.
For the calculation of the mean distance to the central symmetry axis of the NPC, the same parameters were applied as for the corresponding calculations in Table 1.
Normalization of values for non-diametric perpendicular section planes through NPCs as described in Figure 3B. See also considerations in Supplemental Figure S7.
Values presented are from only one of two highly similar data sets that were obtained with different batches of Tpr 636-655 antibodies from two guinea pigs. Normalized mean values for the second data set were -33.5 nm (distance to NE midplane) and 36.2 nm (distance to NPC-axis; n = 61), respectively.
Mean values for Tpr (636-655) that only include gold grains up to 50 nm on the nuclear side (n = 77) are -24.9 nm (nonnormalized) and -27.4 nm (normalized). Mean values for grains up to 100 nm on the nuclear side are -32.0 nm (nonnormalized, n = 92) and -34.6 nm (normalized, n = 93).