Table 3. PRP application in vitro.
| Cell type | Application to culture | Outcome | Reference |
|---|---|---|---|
| Bone | |||
| Mouse BMSCs | PRP-BMP2 genetically modified MSCs(2.5%–5% PRP for in vitro and 10% for ex vivo) | Improve BMSCs proliferation and differentiation | 177 |
| Synovial fluid MSCs | SF-MSCs-alginate system (20%–50% PRP) 50% demonstrated highest activity | Promoted MSCs proliferation and chondrocyte differentiation | 178 |
| MSCs derived from canine umbilical cord | Co-cultured with PRP and demineralized bone matrix. | Induced osteogenesis in vitro of MSCs derived from the umbilical cord | 179 |
| BMSCs | BMSCs+PRP | Increased MSCs proliferation and differentiation | 176 |
| Human ASCs | Combination of chitosan-PRP and either Nano-hydroxyapatite or tricalcium phosphate | Enhanced MSCs proliferation and differentiation | 180 |
| Human DPSCs | PRP (1%, 5%, 10%, 20%) | 1%–10% PRP showed significant effect of promoting hDPSC osteogenic differentiation | 181 |
| DPSCs | DPSC+PRP | Increased DPSCs proliferation at 0.5% and 1% PRP concentration but decreased at 5% concentration. Long-term treatment with 1% PRP showed most significant enhancement at 96 h. | 182 |
| Human ASCs | culture medium containing 10 mL·L−1 PRP | Increased ASCs osteogenic differentiation and proliferation | 183 |
| Human MSCs | calcium phosphate scaffolds+PRP | Increased MSCs osteogenic differentiation and proliferation | 184 |
| Cartilage | |||
| Human MSCs | 10% PRP | Increased MSCs proliferation and chondrogenesis | 173 |
| Human BMSCs | 10% PRP | Increased MSCs migration and proliferation | 185 |
| Human ASCs | 10% PRP | Increased ASCs proliferation and preservation of immunophenotype and differentiation | 186 |
| Mouse MDSCs | 10% PRP | Upregulation of type II collagen and improved MDSCs proliferation | 187 |
| Nude rat MDSCs | PRP+VEGF antagonist+BMP-4 | Type II collagen increased | 188 |
| Sheep MDSCs | PRP | Increased MDSCs proliferation | 189 |
| Human ADSCs | PRP and insulin in 3D collagen scaffold | Increased chondrogenic and osteogenic differentiation of ASCs | 190 |
Abbrevations: BMSC, bone marrow-derived mesenchymal stem cell; DPSC, dental pulp stem cell; MDSC, muscle-derived stem cell; MSC, mesenchymal stem cell; PRP, platelet-rich plasma; VEGF, vascular endothelial growth factor.