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. 2014 Jul 11;6(4):212–218. doi: 10.1038/ijos.2014.32

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Copyright © 2014 West China School of Stomatology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permissing from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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In vivo tumorigenesis assay. Varying numbers of sphere-forming-like cells from the VX2 rabbit buccal carcinoma were subcutaneously injected into nude mice. Representative image showing tumour xenograft formation (a) and representative micrograph showing the histological differentiation of the tumour xenograft (b, haematoxylin–eosin stain), which was similar to the original VX2 rabbit buccal carcinoma. As few as 10³ cells produced visible tumour formation within 7 weeks post-transplantation (c). Different numbers of primary culture cells from the VX2 rabbit buccal carcinoma were subcutaneously injected into nude mice. Representative image showing a lack of visible tumour xenograft formation (d) for cell concentrations up to 10⁴ (e).