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. 2014 Jul 25;6(4):195–204. doi: 10.1038/ijos.2014.38

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Copyright © 2014 West China School of Stomatology

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/

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CLSM images (maximum projection) of 24-h S. mutans biofilms on enamel slides. (a–c) Vitality (live/dead) of biofilms grown in the control medium, sucrose medium, and xylitol medium; (d–f) respiratory activity (CTC) of biofilms grown in the control medium, sucrose medium and xylitol medium; (g–i) EPS production (Con A) in biofilms grown in the control medium, sucrose medium and xylitol medium. Con A, concanavalin A; CTC, 5-cyano-2,3-ditolyl tetrazolium chloride; EPS, extracellular polysaccharide.