Table 1. Primers for housekeeping genes (GAPDH and β-actin), MMPs and TIMPs for semi-quantitative PCR.
| mRNA | Primer pairs |
|---|---|
| GAPDH (233 bp) | Forward: GGTGAAGGTCGGTGTGAACG |
| Reverse: CTCGCTCCTGGAAGATGGTG | |
| β-actin (266 bp) | Forward: GTCCCTCACCCTCCCAAAAG |
| Reverse: GCTGCCTCAACACCTCAACCC | |
| MMP-1 (106 bp) | Forward: TCATACTACCATCCTGCGACTC |
| Reverse: TCACCTCTAAGCCAAAGAAAGA | |
| MMP-2 (110 bp) | Forward: ATGTGTCTTCCCCTTCACTTTC |
| Reverse: GGTCATCATCGTAGTTGGTTGT | |
| MMP-3 (113 bp) | Forward: AAGGTCTGGGAGGAGGTGAC |
| Reverse: CCATCAAAAGGGACAAAGTCTC | |
| MMP-9 (111 bp) | Forward: CTTCCCCAAAGACCTGAAAAC |
| Reverse: ACTGCTTCTCTCCCATCATCT | |
| TIMP-1 (145 bp) | Forward: CTGGCATCCTCTTGTTGCTATC |
| Reverse: AAGGTGGTCTCGTTGATTTCTG | |
| TIMP-2 (101 bp) | Forward: TCTGAAGTCTGGTAGCCTGTGA |
| Reverse: ACCGTTTCTTTGGGGTTTCT | |
| TIMP-3 (104 bp) | Forward: CAGGGGAGTGTGAGTGTTAGGT |
| Reverse: TGGGGAAGAAGTGTATGCTGTC | |
| TIMP-4 (109 bp) | Forward: CTTGCGATGTGTGCTATGGTAG |
| Reverse: TTGAGACAGTGGGAGTAGGAGAT |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP, metalloproteinase; PCR, polymerase chain reaction; TIMP, tissue inhibitors of metalloproteinase.