Figure 4.

The mod(r) domains of HCRP1 and hVps37B/C interact with Tsg101. (A) GST alone or fused with HCRP1(1–148) or HCRP1(208–397) was immobilized on glutathione-Sepharose beads and incubated with in vitro-translated ³⁵S-labeled Tsg101 for 1 h at 4°C. The beads were then washed and analyzed by SDS-PAGE and fluorography. The input amount is indicated. (B) GST alone or fused with HCRP1 (208–397), hVps37C (146–330), or hVps37B (1–168) was immobilized on glutathione-Sepharose beads and incubated with in vitro-translated ³⁵S-labeled Tsg101 as described in A. The doublet band representing Tsg101 is probably due to translational initiation at alternative positions in vitro (Bache et al., 2003).