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. 2016 Dec 13;6:38899. doi: 10.1038/srep38899

Figure 7. Oxidative stress mediates ethanol and acetaldehyde-induced tight junction disruption and barrier dysfunction.

Figure 7

Caco-2 cells were preincubated with NAC (10 mM), DPI (10 μM) or CsA (5 μM) 30 min prior to incubation with acetaldehyde (200 μM) in the presence of 75 mM ethanol (closed bars; EtOH+AA) or without acetaldehyde or ethanol treatment (gray bars). At 4-hour incubation, TER (A) and unidirectional flux of FITC-inulin (B) were measured. Fixed cell monolayers were stained for occludin (green) and ZO-1 (red) by immunofluorescence method (C). Values are mean ± SEM (n = 6). Asterisks indicate values that are significantly different from corresponding values for control cell monolayers (gray bars). Hash tags indicate values that are significantly different from corresponding values for “None” group.