Figure 1.

(A) PLD physiologically catalyzes hydrolysis of phosphatidylcholine to form PA (top) and can also mediate transphosphatidylation reactions with short-chain primary alcohols (e.g., butanol) to form phosphatidyl alcohols. (B) Two-step approach to image PLD-mediated PA synthesis. Cells are treated with alkynols, resulting in PLD-mediated formation of phosphatidyl alkynols, followed by CuAAC labeling with an azido fluorophore to enable visualization or quantification.