PLD accepts alkynols as substrates for transphosphatidylation. DOPC and Arachis hypogaea PLD were incubated with the indicated alkynol (solid line) or no alkynol (dotted line), followed by lipid extraction, CuAAC labeling with Az488, and HPLC analysis coupled to fluorescence or mass spectrometry detection. Shown are fluorescence traces, with masses indicated next to the curves (obs, observed; exp, expected).