Table 1.
Clinicopathological data of 71 biliary tract cancer patients included in the tissue study. Associations of clinicopathological data with relative DNA methylation of SHOX2 and SEPT9 in tumor tissue
| SHOX2 methylation | SEPT9 methylation | |||
|---|---|---|---|---|
| Clinicopathological data | n | (%) | ||
| p value (n) | p value (n) | |||
| Tumor location | ||||
| GBC | 17 | (24) | ||
| IHC | 25 | (35) | ||
| PHC (Klatskin tumor) | 22 | (31) | ||
| DC | 7 | (10) | ||
| GBC vs. CC | 0.46 (55) | 0.014* (55) | ||
| Gender | ||||
| Male | 42 | (59) | ||
| Female | 29 | (41) | ||
| Male vs. female | 0.10 (55) | 0.14 (55) | ||
| Age at diagnosis | ||||
| ≤60 years | 31 | (44) | ||
| >60 years | 40 | (56) | ||
| Median age [years] | 63 | NA | ||
| Mean age [years] | 62 | NA | ||
| Range [years] | 36–83 | NA | ||
| ≤60 vs. >60 years | 0.57 (55) | 0.031* (55) | ||
| Tumor grade | ||||
| G1 | 5 | (7) | ||
| G2 | 33 | (46.5) | ||
| G3 | 33 | (46.5) | ||
| G1, G2, vs. G3 | 0.22 (55) | 0.68 (55) | ||
| Tumor stage | ||||
| T1 | 10 | (14) | ||
| T2 | 17 | (24) | ||
| T3 | 38 | (53.5) | ||
| T4 | 6 | (8.5) | ||
| T1, T2 vs. T3, T4 | 0.51 (55) | 0.013* (55) | ||
| Lymph node status | ||||
| N0 | 32 | (45.1) | ||
| N1 | 15 | (21.1) | ||
| N2 | 1 | (1.4) | ||
| Unknown | 23 | (32.4) | ||
| N0 vs. N1, N2 | 0.19 (39) | 0.30 (39) | ||
| Venous invasion | ||||
| V0 | 42 | (59) | ||
| V1 | 19 | (27) | ||
| Unknown | 10 | (14) | ||
| V0 vs. V1 | 0.97 (48) | 0.76 (48) | ||
| Lymphatic invasion | ||||
| L0 | 34 | (48) | ||
| L1 | 20 | (28) | ||
| Unknown | 17 | (24) | ||
| L0 vs. L1 | 0.67 (42) | 0.70 (42) | ||
| Perineural invasion | ||||
| Pn0 | 17 | (24) | ||
| Pn1 | 35 | (49) | ||
| Unknown | 19 | (27) | ||
| Pn0 vs. Pn1 | 0.37 (41) | 0.82 (41) | ||
| Distant metastases | ||||
| M0 | 65 | (91.5) | ||
| M1 | 6 | (8.5) | ||
| M0 vs. M1 | 0.32 (55) | 0.36 (55) | ||
| UICC stage | ||||
| UICC I | 4 | (6) | ||
| UICC II | 9 | (13) | ||
| UICC III | 28 | (39) | ||
| UICC IV | 10 | (14) | ||
| Unknown | 20 | (28) | ||
| UICC I, II vs. UICC III, IV | 0.08 (41) | 0.06 (41) | ||
| Surgical margin | ||||
| R0 | 52 | (73) | ||
| R1 | 19 | (27) | ||
| R0 vs. R1 | 0.24 (55) | 0.66 (55) | ||
| Follow-up | ||||
| Follow-up available | 71 | (100) | ||
| Median follow-up [months] | 15 | NA | ||
| Mean follow-up [months] | 23 | NA | ||
| Range [months] | 0–104 | NA | ||
| Deceased | 50 | (70) | ||
| Censored | 21 | (30) | ||
p values refer to the Mann-Whitney U test
*p < 0.05