Table 1. The colistin MIC of E. coli strain BL21 carrying the wild type mcr-1 gene and mutation-containing fragment which encode amino acids 1–541 of the MCR-1 protein.
| E. coli BL21 Strains | Colistin MIC (μg/ml) |
|---|---|
| BL21 | 2 |
| pET15b | 2 |
| pET15b-mcr-1FL* | 8 |
| pET15b-mcr-1FL (T246A) | 2 |
| pET15b-mcr-1FL (T285A) | 2 |
| pET15b-mcr-1FL(D465A) | 2 |
| pET15b-mcr-1FL(H466A) | 2 |
| pET15b-mcr-1FL(H478A) | 2 |
| pET15b-mcr-1FL(H395A) | 8 |
| pET15b-mcr-1FL(N329A) | 8 |
| pET15b-mcr-1FL(S330A) | 8 |
| pET15b-mcr-1FL(D299A) | 8 |
| pET15b-mcr-1FL(E300A) | 8 |
*mcr-1FL represents MCR-1(1–541).