Table 1.
Oligonucleotides used for PCR amplification
| Gen | Oligonucleotides | Genbank ID |
|---|---|---|
| At1r | Fw 5'-AATATTTGGAAACAGCTTGGT-3' Rv 5'-ATGATGATGCAGGTGACTTTG-3' |
[GenBank: NM_030985] |
| Ace | Fw 5'-CCAACAAGACTGCCACCTG-3' Rv 5'-GTACTGGTGACATCGAGGTTG-3' |
[GenBank: NM_012544] |
| B1r | Fw 5'-AGCATCTTCCTGGTGGTGG-3' Rv 5'-CCAGCAGACCAGGAAGGAG-3' |
[GenBank: NM_030851] |
| Klk-1 | Fw 5'-CCCTCACCCTGACTTCAAC-3' Rv 5'-TCACACACTGGAGCTCATC-3' |
[GenBank: 001005382] |
| Nrf2 | Fw 5'-TGCCTTCCTCTGCTGCCATTAG -3' Rv 5'-ATGCTCGGCTGGGACTTGTG -3' |
From Applied Biosystems |
| Hmox1 | Fw 5'-AGGGAAGGCTTTAAGCTGGTGATG-3' Rv 5'-CCTGCCAGTGGGGCCCATAC-3' |
From Applied Biosystems |
| Sod2 | Fw 5'-TCCCTATCTCTGTGGTGGTGATG-3' Rv 5'-TATCCTGGTCATAGCCGAAGTCTC-3' |
From Applied Biosystems |
| Col3a1 | Fw 5'-AGGGTGATCGTGGTGAAAA-3' Rv 5'-TCCTCGATGTCCTTTGATG-3' |
[GenBank: NM_032085] |
| 18S | Fw 5'-GCAGCTAGGAATAATGGAATA-3' Rv 5'-GACTTTCGTTCTTGATTAATGA-3' |
[GenBank: NR_046237] |
The gene name, the sequence of the forward (Fw) and reverse (Rv) oligonucleotides, and the Genbank ID or commercial source are shown