Fig. 1.

Creating arrays of several ribbons with a custom-built substrate holder. Ultramicrotome with substrate holder attached (a), jumbo diamond knife with large boat that can harbour substrates up to microscope slide size (b). 12 ribbons (about 270 sections total) attached to substrate, still floating on water surface in knife boat (c) and the same sections dried onto a piece of silicon wafer after lift-out (d). For correlative light and electron microscopy ITO-coated, transparent coverslips (e) are the substrate of choice. Section thickness can range from ultrathin (c-e) to semi-thin (f). Scale bars: 10 mm in C, D, E, 1 mm in F