Fig. 1.

Laminin 521/511 and MG support superior differentiation and proliferation in freshly isolated satellite cells. a Differentiation of freshly isolated satellite cells (Dba/2J), myosin heavy chain (green), and Hoechst (blue). b Quantification of differentiation via mean MHC calculated area (Dba/2J). c Quantification of total nuclei per well via Hoechst nuclei count (Dba/2J). Quantification of differentiation via MHC calculated area from freshly isolated C57/BL6 (d) and C57/BL10 (e). Error bars represent means ±S.D. Scale bar represents 200 μM. N = 3 biological replicates per group. Statistical significance was determined by one-way ANOVA with Bonferroni correction. Significance is annotated as less than .05 (*), less than .01 (**), less than .001 (***), and less than .0001 (****). All comparisons were conducted using laminin 521 as control