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. 2016 Dec 13;6:44. doi: 10.1186/s13395-016-0116-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Expanded cells are purely myogenic and exhibit similar Pax7/MyoD expression. a Cells display greater than 99% positive expression of satellite cell/myoblast marker integrin α7—PE. Twenty thousand cells were analyzed for each substrate. b, c Cells on all substrates maintain similar expression regarding proportion of positive cells and staining intensity for both Pax7 and MyoD. N = 6 technical replicates for each group from one biological sample. Significance was determined by one-way ANOVA with Bonferroni correction. Significance is annotated as less than .05 (*), and less than .001 (***). All comparisons were conducted using laminin 521 as control