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. Author manuscript; available in PMC: 2016 Dec 13.
Published in final edited form as: Methods Enzymol. 2014 Dec 4;550:251–265. doi: 10.1016/bs.mie.2014.10.034

Figure 3. Sequence of Phsp60-ribo regulatory element.

Figure 3

The 5’UTR of Phsp60 is truncated as described (Topp et al, 2010). Restriction sites KpnI or XbaI/AclI can be used to replace Phsp60 with a different promoter.