Figure 5. Relative transcriptional levels of E. coli KRX genes found in P. pastoris strains.

Sfmf (fimbrial protein) in JPS086, ychA (adhesin AidA precursor) in JPS094, ybaA (signalling protein) in JPS300 and finO (fertility inhibitor) in JPS496. Samples for qRT-PCR experiments were taken in the exponential growth phase from cells grown in MD-medium. ACT1 was used as housekeeping gene and values were normalized relative to the expression level of the ACT1 gene in each sample. Error bars represent the standard deviation with n = 3.