Table 1. Characteristics of P. pastoris strains displaying an abnormal colony morphology, determined as detailed in Schwarzhans et al. 24.
Strain | Mut-Phenotype | GCN | ExpLvl |
---|---|---|---|
JPS014 | Mut+ | 0.7 | 0.3 |
JPS086 | Mut+ | 0.4 | 1.1 |
JPS094 | Mut+ | 2.5 | 2.6 |
JPS165 | Mut+ | 0.9 | 1.0 |
JPS247 | Mut+ | 0.9 | 0.6 |
JPS277 | MutS | 0.9 | 0.9 |
JPS280 | Mut+ | 2.4 | 3.5 |
JPS300 | Mut+ | 0.5 | 0.5 |
JPS315 | MutS | 0.9 | 0.8 |
JPS336 | Mut+ | 1.0 | 0.1 |
JPS347 | Mut+ | 0.3 | 1.1 |
JPS379 | Mut+ | 0.1 | 0.0 |
JPS386 | Mut+ | 5.2 | 1.2 |
JPS394 | Mut+ | 0.6 | 0.8 |
JPS495 | Mut+ | 0.1 | 1.7 |
JPS496 | Mut+ | 0.5 | 0.5 |
JPS581 | Mut+ | 0.8 | 1.4 |
JPS603 | Mut+ | 3.1 | 0.6 |
JPS604 | Mut+ | 0.2 | 0.0 |
JPS626 | Mut+ | 0.6 | 0.7 |
JPS636 | Mut+ | 0.8 | 1.0 |
JPS733 | Mut+ | 0.6 | 0.3 |
JPS749 | Mut+ | 0.7 | 0.4 |
JPS768 | Mut+ | 0.6 | 0.5 |
JPS804 | Mut+ | 0.7 | 0.6 |
JPS835 | Mut+ | 0.8 | 0.8 |
A gene copy number (GCN) <1 can result from e.g. a reduced PCR efficiency during qPCR, but is still indicative of a single copy of the GFP cassette. The GFP expression level (ExpLvl) was normalized against a reference MutS strain with one copy of the cassette. Underlined strains were selected for genome sequencing.