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. 2016 Dec 13;6:38952. doi: 10.1038/srep38952

Table 1. Characteristics of P. pastoris strains displaying an abnormal colony morphology, determined as detailed in Schwarzhans et al. 24.

Strain Mut-Phenotype GCN ExpLvl
JPS014 Mut+ 0.7 0.3
JPS086 Mut+ 0.4 1.1
JPS094 Mut+ 2.5 2.6
JPS165 Mut+ 0.9 1.0
JPS247 Mut+ 0.9 0.6
JPS277 MutS 0.9 0.9
JPS280 Mut+ 2.4 3.5
JPS300 Mut+ 0.5 0.5
JPS315 MutS 0.9 0.8
JPS336 Mut+ 1.0 0.1
JPS347 Mut+ 0.3 1.1
JPS379 Mut+ 0.1 0.0
JPS386 Mut+ 5.2 1.2
JPS394 Mut+ 0.6 0.8
JPS495 Mut+ 0.1 1.7
JPS496 Mut+ 0.5 0.5
JPS581 Mut+ 0.8 1.4
JPS603 Mut+ 3.1 0.6
JPS604 Mut+ 0.2 0.0
JPS626 Mut+ 0.6 0.7
JPS636 Mut+ 0.8 1.0
JPS733 Mut+ 0.6 0.3
JPS749 Mut+ 0.7 0.4
JPS768 Mut+ 0.6 0.5
JPS804 Mut+ 0.7 0.6
JPS835 Mut+ 0.8 0.8

A gene copy number (GCN) <1 can result from e.g. a reduced PCR efficiency during qPCR, but is still indicative of a single copy of the GFP cassette. The GFP expression level (ExpLvl) was normalized against a reference MutS strain with one copy of the cassette. Underlined strains were selected for genome sequencing.