MXR1-FW |
GAGAAGGATAGGATCACGGTGGCC |
MXR1 locus amplification |
MXR1-RV |
GCGCCAGAGACATTCTACAGGGAG |
ADH-FW |
CCCTTTATCACCAACTTGAACTCTCACATTCCCCC |
Amplification of Adhesin AidA precursor ychA found in JPS094 |
ADH-RV |
GGCCTGGTGCAACAGGATGGATTAATATTTTTAATGG |
FIM-FW |
CTCAAATATCTTTAATAGCACCAATAACCAGCCAGGG |
Amplification of Adhesin fragment fimH and Fimbrial protein found in JPS086 |
FIM-RV |
CATCTGCCTAAATCTACCGTCTTATCAATATCCGC |
ACT1-FW |
GGTGTGGTGCCAGATCTTTT |
qRT-PCR; Housekeeping gene ACT1 in P. pastoris; Amplicon = 181 bp |
ACT1-RV |
AGTGTTCCCATCGGTCGTAG |
sfmF-FW |
TACTGAACGCTGGCGATACC |
qRT-PCR; sfmF in JPS086 and E. coli KRX, Amplicon = 163 bp |
sfmF-RV |
AATTCGATGGCGACGGTTTG |
ychA-FW |
GCAGCGTGCCATTATTACCG |
qRT-PCR; ychA in JPS094 and E. coli KRX, Amplicon = 189 bp |
ychA-RV |
TTGGGCAATCTGATGCACCT |
ybaA-FW |
TCGGAACGGAACGGATAAGAC |
qRT-PCR; ybaA in JPS300 and E. coli KRX, Amplicon = 160 bp |
ybaA-RV |
TGCAAGTGTGAAAGCTCAGCA |
finO-FW |
AATGTCACCACGCCACCAAA |
qRT-PCR; finO in JPS496 and E. coli KRX, Amplicon = 159 bp |
finO-RV |
CTTCAGGGTGTTCACGGCAT |