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. 2016 Dec 13;6:38952. doi: 10.1038/srep38952

Table 2. Primers used for PCR assays and qRT-PCR.

Primer name Sequence Purpose
MXR1-FW GAGAAGGATAGGATCACGGTGGCC MXR1 locus amplification
MXR1-RV GCGCCAGAGACATTCTACAGGGAG
ADH-FW CCCTTTATCACCAACTTGAACTCTCACATTCCCCC Amplification of Adhesin AidA precursor ychA found in JPS094
ADH-RV GGCCTGGTGCAACAGGATGGATTAATATTTTTAATGG
FIM-FW CTCAAATATCTTTAATAGCACCAATAACCAGCCAGGG Amplification of Adhesin fragment fimH and Fimbrial protein found in JPS086
FIM-RV CATCTGCCTAAATCTACCGTCTTATCAATATCCGC
ACT1-FW GGTGTGGTGCCAGATCTTTT qRT-PCR; Housekeeping gene ACT1 in P. pastoris; Amplicon = 181 bp
ACT1-RV AGTGTTCCCATCGGTCGTAG
sfmF-FW TACTGAACGCTGGCGATACC qRT-PCR; sfmF in JPS086 and E. coli KRX, Amplicon = 163 bp
sfmF-RV AATTCGATGGCGACGGTTTG
ychA-FW GCAGCGTGCCATTATTACCG qRT-PCR; ychA in JPS094 and E. coli KRX, Amplicon = 189 bp
ychA-RV TTGGGCAATCTGATGCACCT
ybaA-FW TCGGAACGGAACGGATAAGAC qRT-PCR; ybaA in JPS300 and E. coli KRX, Amplicon = 160 bp
ybaA-RV TGCAAGTGTGAAAGCTCAGCA
finO-FW AATGTCACCACGCCACCAAA qRT-PCR; finO in JPS496 and E. coli KRX, Amplicon = 159 bp
finO-RV CTTCAGGGTGTTCACGGCAT