Figure 1. The expression of peroxiredoxin-3 (PRDX3) was decreased in placentas from intrahepatic cholestasis of pregnancy (ICP) patients.

(A) Immunoblot assay for detecting PRDX3 protein level in placentas from normal pregnancies and ICP patients. Full-length blots were presented in Supplementary Fig. S7. (B) Relative protein level of PRDX3 through densitometric analysis of immunoblot results from (A) with normalisation to β-Actin (n = 12 in each group, ***p < 0.001, Student’s t test). (C) Immunohistochemistry analysis of PRDX3 in trophoblasts of placental tissues. Immunostaining intensity was showed as 1+ (weak), 2+ (moderate) or 3+ (strong). Original magnification 200x, bar: 100 μm. (D) Statistically analysis of immunostaining signal of PRDX3 in placentas (n = 46 for normal, n = 70 for ICP, ***p < 0.001, Fisher’s exact test).