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. 2016 Dec 13;6:38999. doi: 10.1038/srep38999

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Spinal cord, (B) spleen and (C) dLN tissues were harvested from naïve C57BL/6 mice or at EAE onset, peak and resolution stages of MOG_35-55 immunised mice. RNA was isolated and reverse transcribed to allow analysis of MKP-2 mRNA expression by quantitative PCR. (D) Spleen and (E) dLN tissues were harvested from EAE peak mice and single cell suspensions prepared and cultured with or without MOG_35-55 for the times indicated. Cells were then harvested for analysis of MKP-2 mRNA expression by quantitative PCR. Results show changes in MKP-2 mRNA expression relative to naïve tissue or unstimulated cells using the 2-ΔΔCT method. (A–C) Each symbol represents an individual mouse and bar indicates the average value of relative MKP-2 expression in each group. (D–E) Graphs show data from two independent experiments, n = 8. *P < 0.05.