Figure 5. CD4⁺ T cells of non-immunised MKP-2^−/− mice have reduced cell proliferation and cytokine production.

CD4⁺ T cells were isolated from spleens of MKP-2^+/+ and MKP-2^−/− mice, and then activated in culture with anti-CD3 and anti-CD28 antibodies. (A) After 48 and 96 hours, T cell proliferation was measured by MTT assay. (B) Cell supernatants were also collected after 72 hours of culture and then cytokine levels were measured by ELISA. Values represent mean±SEM of three independent experiments, *P < 0.05; **p < 0.01.