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. 2016 Dec 13;6:38999. doi: 10.1038/srep38999

Figure 7. MKP-2 is important for NO production by macrophages.

Figure 7

(A) Spleen and dLNs were harvested at EAE peak and disrupted to form individual cell suspensions and cells stained for CD11b expression. FACS plots are representative of at least 9 mice per group from three independent experiments. Graphs show combined data from three independent experiments, bars represent mean ± SEM. (B) Whole blood was harvested at EAE peak and resolution stages and nitrite concentrations determined by Griess Assay. Graphs show mean ± SEM of two independent experiments (n = 7 MKP-2+/+; n = 8 MKP-2−/−). (C) BMMs were generated from the bone marrow from MKP-2+/+ and MKP-2−/− mice. Cells were stimulated with or without LPS (100 ng/ml) and culture supernatant was collected after 24 hours and nitrite concentrations determined by Griess Assay. Values represent mean ± SEM of three independent experiments. (D) BMMs were stimulated with or without LPS (100 ng/ml), CpG (1 μM) or Poly(I:C) (20 μg/ml) for 24 hours and NOS2 expression was analysed by flow cytometry following initial gating on CD11b+ cells. FACS plots are representative of three independent experiments of BMMs either unstimulated or activated by LPS. Graphs show combined data from three independent experiments, bars represent mean ± SEM. *P < 0.05, **P < 0.01.