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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 May 19;36(7):1350–1355. doi: 10.1161/ATVBAHA.116.307182

Figure 2.

Figure 2

As predicted by motif analysis (see suppl figure I), EMSA assays revealed that HNF4A recombinant protein directly interacts and displays a higher affinity for the reference A allele of the TMEM241 eQTL SNP rs17259126 (figure 2A) although no super shift was obtained when using HepG2 nuclear extract and anti-HNF4A (figure 2B). a) HNF4A isolate. b) HepG2 hepatoma nuclear extract. HNF4A (HNF4A isolated protein), unlabeled probe (UP), labeled probe (LP), nuclear extract (NE), and anti-HNF4A (anti-HNF4A).