Figure 6.
Treatment with myricetin and spinacine (5) increases cellular levels of caspase 8 and p53. (a) SiHa cells (1 × 106 per well) were seeded into a six-well plate and allowed to incubate overnight. The indicated concentrations of myricetin and spinacine were added, and then cells were incubated for 24 h. Cells were then washed in 1 × PBS and then harvested, and the resulting level of caspase 8 was measured by immunoblot. (b) SiHa and C33A cells (1 × 106 per well) were seeded into a six-well plate and allowed to incubate overnight. In all, 200 μM of myricetin and 100 μM spinacine were added together with 4 μg/ml mitomycin C and incubated for 24 h. The resulting level of p53 was measured by enzyme-linked immunosorbent assay, and the level of p53 found in untreated cells was set at 100%. Experiments were performed in triplicate, and error bars indicate the S.D.