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. 2016 Feb 15;5(2):e195. doi: 10.1038/oncsis.2015.42

Figure 2.

Figure 2

Effect of PI3K (LY294002) and MAPK (U0126) inhibitors and an AMPK activator (AICAR) on FASN expression in LNCaP and C4-2 cells. LNCaP and C4-2 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) with and without 5 μM LY294002, 0.5 μM U0126 (a–c), or AICAR (d) at the indicated concentration for 24 h. (a) An equal amount of protein from the cells and conditioned medium was subjected to anti-human-FASN antibody. Total RNA was extracted from the cells and FASN (b) and SREBP-1 (c) mRNA levels were measured by quantitative reverse transcription–PCR, and compared with those of untreated cells. **P<0.01. (d) The protein from the cultured cells and the supernatant was subjected to anti-AMPK, anti-P-AMPK, anti-FASN, anti-SREBP-1 and anti-beta-actin.