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. 2016 Feb 29;5(2):e201. doi: 10.1038/oncsis.2016.14

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Flow cytometry and confocal microscopy analysis of P17 binding to various cell lines undergoing drug-induced apoptosis. (a) Flow cytometry quantification of the cellular fluorescence in cells treated with apoptosis inducers following incubation with FITC-P17 versus control samples shown via mean fluorescence intensity. Percentage of apoptosis was determined using Annexin V/PI staining and expressed as the proportion of Annexin V-positive cell counts in total counts. Data are presented as means±s.d. (n=3). ***P<0.001. (b) Confocal microscopy observation of FITC-P17 (green) accumulating in cells undergoing apoptosis. Nuclei stained blue with DAPI. Scale bars, 50 μm (THP-1); 100 μm (other cell lines).