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. 2016 Feb 29;5(2):e201. doi: 10.1038/oncsis.2016.14

Figure 6.

Figure 6

Comparison of P17 with conventional apoptosis detection agents in apoptotic cells. TRAIL-treated and control Jurkat cells were incubated with FITC-P17 for 30 min, and then co-stained with Annexin V (Alexa Fluor 647-conjugated; a), cleaved caspase-3 antibody (Alexa Fluor 647-conjugated; b) or PI (c), before analysis on the flow cytometer. All the samples were plotted as P17 on the x axis versus other apoptosis-imaging agents' fluorescence on the y axis. The quadrants Q in (a) and (b) were defined as Q1=live (P17-negative/Annexin V- or CC3-negative), Q2=necrosis (P17-positive/Annexin V- or CC3-negative), Q3=late apoptosis (P17-negative/Annexin V- or CC3-positive) and Q4=early apoptosis (P17-positive/Annexin V- or CC3-negative). The quadrants in (c) were defined as Q1=live (P17-negative/PI-negative) and Q3=late apoptosis (P17-positive/PI-positive).