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Effect of media choice on conjugation efficiency of C. difficile strain R20291 after heat treatment. BHI, BHIS, TY and Brazier’s agar were tested in 8 h conjugations following a 52 °C, 5 min heat treatment. Use of Brazier’s agar resulted in the recovery of no transconjugants. Each bar represents the mean and standard deviation of data collected from experiments performed in triplicate using biological duplicates.
