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. Author manuscript; available in PMC: 2017 Aug 30.
Published in final edited form as: Cell Rep. 2016 Aug 18;16(9):2428–2441. doi: 10.1016/j.celrep.2016.07.058

Figure 3. p38/ERK Signaling Promotes GATA-2 Chromatin Occupancy and Chromatin Remodeling.

Figure 3

(A) Gata2 locus map. Numbers represent distance to mouse 1S transcription start site. 1G is another GATA2 transcription start site. Quantitative ChIP analysis of GATA-2 occupancy in Kasumi-1 cells treated with 40 μM SB203580 or 20 μM U0126 is shown (n = 4, mean ± SE, *p < 0.05 and **p < 0.01). The western blot (anti-GATA-2 antibody) (inset) illustrates GATA-2 expression in representative samples used for ChIP.

(B) Quantitative ChIP analysis of GATA-2 occupancy at GATA-2 target genes in Kasumi-1 cells treated with 40 μM SB203580 or 20 μM U0126 is shown (n = 4, mean ± SE, *p < 0.05).

(C) Quantitative FAIRE analysis of chromatin accessibility in Kasumi-1 cells treated with 40 μM SB203580 or 20 μM U0126 is shown (n = 4, mean ± SE, *p < 0.05 and **p < 0.01).

(D) Kinetics of GATA-2 expression and phosphorylation are shown (n = 3, mean ± SE, *p < 0.05).

(E) GATA-2 occupancy at the −77 kb site during 40 μM SB203580 treatment is shown (n = 3, mean ± SE, *p < 0.05).

(F) Kinetics of GATA-2 protein expression and phosphorylation are shown (n = 3, mean ± SE, *p < 0.05).

(G) GATA-2 occupancy at the −77 kb site during 20 μM U0126 treatment is shown (n = 3, mean ± SE, *p < 0.05).

(H) Left: rescue assay. Right: qRT-PCR analysis of IL1B and CXCL2 in Kasumi-1 cells expressing GATA-2 or control vector with or without 10 μM SB203580 is shown (n = 4; mean ± SE; *p < 0.05; PI, preimmune).

See also Figure S3.